교수소개

유혜현 부교수

약학과
연구분야 : 약품분석

연락처 : 031-400-5804

1. 학력
-1999.2 서울대학교 약학대학 약학사 
-2001.2 서울대학교 약학대학 약학석사 (약품분석 전공)
-2005.2 서울대학교 약학대학 약학박사 (약품분석 전공)

2. 경력
-2001 미국 일리노이대 약대, 객원연구원
-2004 일본 도야마대 화한약연구소, 객원연구원
-2005~2010 한국과학기술연구원 도핑콘트롤센터 연구원, 선임연구원
-2008~2010 고려대학교 화학과 겸임교수
-2008~2010 과학기술연합대학원대학교 (UST) 겸임교수 (생물분석과학)
-2010~ 한양대학교 약학대학 조교수

3. 관심연구분야
- 기기분석(HPLC, LC/MS)을 이용한 생체시료 중 약물 및 대사체 분석법 확립
- 후보약물의 전임상 ADME 연구
- 약물 대사 profiling 및 대사체 구조 규명 
- CYP reaction phenotyping 
- 약물 상호 작용 규명 (CYP inhibition and induction)
- 천연물 PK 연구 및 herb-drug interaction

4. 발표논문 
(1) Lee SK, Kim DH, Yoo HH. Comparative metabolism of sildenafil in liver microsomes of different species by using LC/MS-based multivariate analysis. J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Oct 15;879(28):3005-11
(2) Yoo HH, Kim NS, Kim MJ, Shin D, Shin JG, Kim DH. Enantioselective carbonyl reduction of eperisone in human liver microsomes. Xenobiotica. 2011 Sep;41(9):758-63
(3) Jeon BW, Yoo HH, Jeong ES, Kim HJ, Jin C, Kim DH, Lee J. LC-ESI/MS/MS method for rapid screening and confirmation of 44 exogenous anabolic steroids in human urine. Anal Bioanal Chem. 2011 Sep;401(4):1353-63
(4) Lee KM, Kim HJ, Jeong ES, Yoo HH, Kwon OS, Jin C, Kim DH, Lee J. Simple and accurate quantitative analysis of seven prohibited threshold substances in human urine by liquid chromatography/tandem mass spectrometry in doping control. Rapid Commun Mass Spectrom. 2011 Aug 30;25(16):2261-7
(5) Jin MJ, Jin C, Kim JY, In MK, Kwon OS, Yoo HH. A quantitative method for simultaneous determination of 5-methoxy-N,N-diisopropyltryptamine and its metabolites in urine using liquid chromatography-electrospray ionization-tandem mass spectrometry. J Forensic Sci. 2011 Jul;56(4):1044-8
(6) Kim Y, Kim UY, In MK, Lee J, Kwon OS, Yoo HH. Hydrophillic Interaction Chromatography-tandem Mass Spectrometry Method for Identification and Quantitation of 5-MeO-DIPT and its Metabolites in Rat Urine. Bull. Korean Chem. Soc. 2011; 32(4): 1158-1164.
(7) Lee BJ, Jo IY, Bu Y, Park JW, Maeng S, Kang H, Jang W, Hwang DS, Lee W, Min K, Kim JI, Yoo HH, Lew JH. Antiplatelet effects of Spatholobus suberectus via inhibition of the glycoprotein IIb/IIIa receptor. J Ethnopharmacol. 2011 Mar 24;134(2):460-7
(8) Lee SK, Kim SH, Kim HJ, Yoo HH, Kwon OS, In MK, Jin C, Kim DH, Lee J. Simultaneous qualitative and quantitative method using liquid chromatography selected reaction monitoring-triggered quantitation-enhanced data-dependent tandem mass spectrometry for the identification and classification of amphetamine-type stimulant abusers in human urine. Rapid Commun Mass Spectrom. 2010 Nov 15;24(21):3139-45.
(9) Jin MJ, Kim U, Kim IS, Kim Y, Kim DH, Han SB, Kim DH, Kwon OS, Yoo HH*. Effects of Gut Microflora on Pharmacokinetics of Hesperidin: A Study in Conventional and Pseudo Germ-Free Rats. Journal of Toxicology and Environmental Health, Part A 2010;73(21-22):1441-50.
(10) MJ Kang, Hyeim Lee, SM Shin, HH Yoo, OS Kwon, C Jin. Performance Characteristic of the β-Human Chorionic Gonadotropin Assay on the Access 2 for Its Use in Doping Control. Bull. Korean Chem. Soc. 2010; 31 (10): 1-4.
(11) MJ Kang, SM Shin, HH Yoo, OS Kwon, C Jin. Characteristics of IEF Patterns and SDS-PAGE Results of Korean EPO Biosimilars. Bull. Korean Chem. Soc. 2010; 31 (9): 2493-2496.
(12) Lee SK, Kim Y, Jin C, Lee SH, Kang MJ, Jeong TC, Jeong SY, Kim DH, Yoo HH*. Inhibitory Effects of Deoxypodophyllotoxin from Anthriscus sylvestris on Human CYP2C9 and CYP3A4. Planta Med. 2010; 76(7):701-4.
(13) Lee SK, Kim DH, Jin C, Yoo HH*. Determination of Urinary Trimethylamine and Trimethylamine N-oxide by Liquid Chromatography-Tandem Mass Spectrometry Using Mixed-Mode Stationary Phases. Bull. Korean Chem. Soc. 2010; 31 (2): 483-486.
(14) Bhuiyan MI, Islam MN, Jung SY, Yoo HH, Lee YS, Jin C. Involvement of ceramide in ischemic tolerance induced by preconditioning with sublethal oxygen-glucose deprivation in primary cultured cortical neurons of rats. Biol Pharm Bull. 2010; 33(1):11-7.
(15) Islam MN, Nam JW, Lee J, Seo EK, Han SB, Kim DH, Jin C, Yoo HH*. A Validated LC Method for Simultaneous Determination of Phenolic, Coumarin and Phthalide Compounds in the Ethanolic Extract of Angelica tenuissima. Chromatographia 2009; 70: 1079-85.
(16) Lee SK, Kang MJ, Jin C, In MK, Kim DH, Yoo HH*. Flavin-containing monooxygenase 1-catalysed N,N-dimethylamphetamine N-oxidation. Xenobiotica. 2009; 39(9): 680-6.
(17) Chung HJ, Kim NS, Kim EJ, Kim TK, Ryu KH, Lee BY, Kim DH, Jin C, Yoo HH*. Negligible Effect of Ginkgo Biloba Extract on the Pharmacokinetics of Cilostazol. Biomolecules & Therapeutics, 17(3), 311-317 (2009).
(18) Islam MN, Yoo HH*, Sung CK, Dong MS, Park YI, Jin C, Kim DH. Simultaneous determination of phenolic acids and phthalide compounds by liquid chromatography for quality assessment of Rhizoma cnidii. J AOAC Int. 2009 Mar-Apr;92(2):375-81.
(19) Yoo HH*, Kim NS, Lee J, Sohn DR, Jin C, Kim DH. Characterization of human cytochrome P450 enzymes involved in the biotransformation of eperisone. Xenobiotica. 2009 Jan;39(1):1-10.
(20) Lee SK, Kim Y, Suh S, Suh YJ, In MK, Kim DH, Jin C, Yoo HH*. Detox agents do not affect the pharmacokinetics of methamphetamine in the rat. Forensic Sci Int. 2009 Apr 15;186(1-3):e21-4.
(21) Yoo HH, Yoon HJ, Shin HJ, Lee SH, Yoon HR. Characterization of Plasma Carnitine Level in Obese Adolescent Korean Women. Biomolecules & Therapeutics, 17(2), 181-187 (2009).
(22) Islam MN, Lee SK, Jeong SY, Kim DH, Jin C, Yoo HH*. Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Herba Epimedii by HPLC. Bull. Korean Chem. Soc. 2009, Vol. 30, No. 1: 137-144.
(23) Lee SK, Kim Y, Kim TK, Im GJ, Lee BY, Kim DH, Jin C, Yoo HH*. Determination of mirodenafil and sildenafil in the plasma and corpus cavernous of SD male rats. J Pharm Biomed Anal. 2009 Feb 20; 49(2):513-8.
(24) Islam MN, Yoo HH*, Lee J, Nam JW, Seo EK, Jin C, Kim DH. Simultaneous determination of bioactive xanthone glycosides and norlignans from ethanolic extract of Anemarrhena asphodeloides by liquid chromatography. J AOAC Int. 2008 Nov-Dec;91(6):1271-7.
(25) Kim JY, Cheong JC, Ko BJ, Lee SK, Yoo HH, Jin C, In MK. Simultaneous determination of methamphetamine, 3,4-methylenedioxy-N-methylamphetamine, 3,4-methylenedioxy-N-ethylamphetamine, N,N-dimethylamphetamine, and their metabolites in urine by liquid chromatography-electrospray ionization-tandem mass spectrometry. Arch Pharm Res. 2008 Dec;31(12):1644-51.
(26) Lee SK, Lee DJ, Yoo HH, Kim JH, Seo YM, Shin S, Choi JH, Jeon TW, Kang MJ, Jeong TC. Identification of glutathione conjugates of 1-bromopentane and its hepatotoxicity in female BALB/c mice. Arch Pharm Res. 2008 Oct;31(10):1317-23
(27) Yoo HH*, Lee SK, Lim SY, Kim Y, Kang MJ, Kim EJ, Park YH, Im GJ, Lee BY, Kim DH. LC-MS/MS method for determination of hederacolchiside E, a neuroactive saponin from Pulsatilla koreana extract in rat plasma for pharmacokinetic study. J Pharm Biomed Anal. 2008 Dec 15;48(5):1425-9.
(28) Yoo HH, Lee SH, Jin C, Kim DH. Mechanism-based inactivation of cytochrome P450 3A4 by methylenedioxyphenyl lignans from Acanthopanax chiisanensis. Planta Med. 2008 Jun;74(8):822-7.
(29) Yoo HH, Chung HJ, Lee J, Lee CS, Kang MJ, Kim DH. Enzymatic C-demethylation of 1-[2-(5-tert-butyl-[1,3,4] oxadiazole-2-carbonyl)-4-fluoro-pyrrolidin-1-yl]-2-(2-hydroxy-1,1-dimethyl-ethylamino)-ethanone (LC15-0133) in rat liver microsomes. Drug Metab Dispos. 2008 Mar;36(3):485-9.
(30) Lee SK, Jun IH, Yoo HH, Kim JH, Seo YM, Kang MJ, Lee SH, Jeong TC, Kim DH. Characterization of in vitro metabolites of deoxypodophyllotoxin in human and rat liver microsomes using liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom. 2008;22(1):52-8.
(31) Islam NM, Yoo HH, Lee MW, Dong MS, Park YI, Jeong HS, Kim DH. Simultaneous quantitation of five flavonoid glycosides in Herba Epimedii by high-performance liquid chromatography-tandem mass spectrometry. Phytochem Anal. 2008 Jan-Feb;19(1):71-7.
(32) Yoo HH*, Lee M, Chung HJ, Lee SK, Kim DH. Effects of diosmin, a flavonoid glycoside in citrus fruits, on P-glycoprotein-mediated drug efflux in human intestinal Caco-2 cells. J Agric Food Chem. 2007 Sep 5;55(18):7620-5.
(33) Son JH, Yoo HH, Kim DH. Activation of de novo synthetic pathway of ceramides is responsible for the initiation of hydrogen peroxide-induced apoptosis in HL-60 cells. J Toxicol Environ Health A. 2007 Aug;70(15-16):1310-8.
(34) Yoo HH, Kim NS, Im GJ, Kim DH. Pharmacokinetics and tissue distribution of a novel PDE5 inhibitor, SK-3530, in rats. Acta Pharmacol Sin. 2007 Aug;28(8):1247-53.
(35) Yoo HH, Lee MW, Kim YC, Yun CH, Kim DH. Mechanism-based inactivation of cytochrome P450 2A6 by decursinol angelate isolated from Angelica Gigas. Drug Metab Dispos. 2007 Oct;35(10):1759-65.
(36) Yoo HH, Park JH, Kwon SW. In vitro cytotoxic activity of some Korean medicinal plants on human cancer cell lines: enhancement in cytotoxicity by heat processing. Phytother Res. 2007 Sep;21(9):900-3.
(37) Yoo HH, Lee M, Lee MW, Lim SY, Shin J, Kim DH. Effects of Schisandra lignans on P-glycoprotein-mediated drug efflux in human intestinal Caco-2. Planta Med. 2007 May;73(5):444-50.
(38) Piao XL, Park JH, Cui J, Kim DH, Yoo HH*. Development of gas chromatographic/mass spectrometry-pattern recognition method for the quality control of Korean Angelica. J Pharm Biomed Anal. 2007 Sep 3;44(5):1163-7.
(39) Kim NS, Yoo HH, Lee MW, Kim HS, Kim DH. Disposition and metabolism of (2S,3S,4R)-N``-cyano-N-(6-amino-3,4-dihydro-3-hydroxy-2-methyl-2-dimethoxy methyl-2H-benzopyran-4-yl)-N`-benzylguanidine, a novel neuroprotective agent for ischemia-reperfusion damage, in rats. Xenobiotica. 2007 May;37(5):534-48.
(40) Kang KS, Kim HY, Baek SH, Yoo HH, Park JH, Yokozawa T. Study on the hydroxyl radical scavenging activity changes of ginseng and ginsenoside-Rb2 by heat processing. Biol Pharm Bull. 2007 Apr;30(4):724-8.
(41) Lee J, Yoo HH, Rhim KJ, Sohn DR, Kim DH. Metabolism and excretion of 5-ethyl-2-{5-[4-(2-hydroxyethyl)piperazine-1-sulfonyl]-2-propoxyphenyl}-7-propyl-3,5-dihydropyrrolo[3,2-d]-pyrimidin-4-one (SK3530) in rats. Rapid Commun Mass Spectrom. 2007;21(7):1139-49.
(42) Cho EJ, Lee YA, Yoo HH, Yokozawa T. Protective effects of broccoli (Brassica oleracea) against oxidative damage in vitro and in vivo. J Nutr Sci Vitaminol (Tokyo). 2006 Dec;52(6):437-44.
(43) Yoo HH, Park JH, Kwon SW. An anti-estrogenic lignan glycoside, tracheloside, from seeds of Carthamus tinctorius. Biosci Biotechnol Biochem. 2006 Nov;70(11):2783-5.
(44) Piao XL, Yoo HH, Kim HY, Kang TL, Hwang GS, Park JH. Estrogenic activity of furanocoumarins isolated from Angelica dahurica. Arch Pharm Res. 2006 Sep;29(9):741-5.
(45) Yoo HH, Son J, Kim DH. Liquid chromatography-tandem mass spectrometric determination of ceramides and related lipid species in cellular extracts. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Nov 7;843(2):327-33.
(46) Yoo HH, Son J, Lee J, Kim NS, Shin M, Kang MJ, Kim DH. The metabolism and excretion of 2-methylaminoethoxycarbonyl-4,4`-dimethoxy-5,6,5`,6`-dimethylenedioxybiphenyl-2`-carboxylic acid (DDB-S) in rats and human. Rapid Commun Mass Spectrom. 2006;20(13):1981-8.
(47) Yoo HH, Kwon SW, Park JH. The cytotoxic saponin from heat-processed Achyranthes fauriei roots. Biol Pharm Bull. 2006 May;29(5):1053-5.
(48) Yoo HH, Yokozawa T, Satoh A, Kang KS, Kim HY. Effects of ginseng on the proliferation of human lung fibroblasts. Am J Chin Med. 2006;34(1):137-46.
(49) Lee J, Yoo BC, Lee HS, Yoo HW, Yoo HH, Kang MJ, Kim DH. Rapid quantitative determination of L-FMAU-TP from human peripheral-blood mononuclear cells of hepatitis B virus-infected patients treated with L-FMAU by ion-pairing, reverse-phase, liquid chromatography/electrospray tandem mass spectrometry. Ther Drug Monit. 2006 Feb;28(1):131-7.
(50) Lee SK, Lee JH, Yoo HH, Kim DH, Jahng Y, Jeong TC. Characterization of human liver cytochrome P450 enzymes involved in the metabolism of rutaecarpine. J Pharm Biomed Anal. 2006 Apr 11;41(1):304-9. 

5. 특허
(1) 당귀 추출물 또는 이로부터 분리한 쿠마린계 화합물을 유효성분으로 함유하는 폐암 예방용 조성물 (등록번호 10-0829122)